Table 1 Advantages and limitations of post-mortem material, animal models, and mono- and co-culture cells models to study human brain development
From: A beginner’s guide on the use of brain organoids for neuroscientists: a systematic review
Advantages | Limitations | |
|---|---|---|
Post-mortem material | Human origin | Fixed temporal representation |
True clinical representation | Material can be scarce | |
Animal models | Allows for over-time studies | Non-human representation |
Systematic model | Requires genetic modifications | |
Scalable | Carry ethical burdens | |
Mono- and co-culture models | Human origin | Usually two-dimensional with limited three-dimensional capabilities |
Scalable | No complex culture conditions | |
Long-term sampling |