A beginner’s guide on the use of brain organoids for neuroscientists: a systematic review

Table 3 Overview of neuronal activity assays performed per organoid model and respective age range

Organoid model	Functional assay	Total analyses	Age range (days)
Cerebral	Calcium [37, 82]	2	NS
	Patch [83, 84]	2	61–121
	Extracellular [66, 85]	2	34- 244
Dorsal forebrain	Calcium [39, 46, 47, 64, 69, 75, 86, 87]	8	45–109
	Patch [22, 39, 64, 81, 86, 88, 89]	7	51–175
	Extracellular [89]	1	165–175
Midbrain	Patch [18]	1	33–84
Midbrain	Extracellular [57]	1	28
Medial ganglionic eminence	Calcium [69]	1	40–50
Medial ganglionic eminence	Patch [69]	1	NS
Spinal cord	Calcium [46]	1	69–96*
Spinal cord	Patch [46]	1	45–75
Thalamus	Calcium [90]	1	49
Thalamus	Patch [90]	1	90–100
Striatum	Calcium [47]	1	90–135*
Striatum	Patch [47]	1	110–170
Brain stem	Patch [63]	1	92
Ventral forebrain	Calcium [22, 87]	2	43–52
Ventral forebrain	Patch [22]	1	125–194*

^*Range includes data from assembloid studies and was determined by the sum of organoid age at time of fusion plus the time of assembloid culture before assay
NS not stated

ISSN: 1757-6512