Table 4 Summary table comparing the cerebral, dorsal forebrain, and ventral forebrain organoid culture protocols
From: A beginner’s guide on the use of brain organoids for neuroscientists: a systematic review
Cerebral organoids | Dorsal forebrain organoids | Ventral forebrain organoids | |
|---|---|---|---|
EB formation | 500–90,000 cells/ EB | 3000–20,000/ EB | 5000–10,000/ EB |
1–7 days | 1–10 days | 1–10 days | |
Neural induction | Small molecules | Small molecules | Small molecules |
Yes (16%), No (84%) | Yes (100%), No (0%) | Yes (100%), No (0%) | |
2–18 days | 4–26 days | 5–24 days | |
Extracellular Matrix | Embedment (93%) | Embedment (8%) | Embedment (0%) |
Liquid ECM (0%) | Liquid ECM (26%) | Liquid ECM (0%) | |
No ECM (7%) | No ECM (66%) | No ECM (100%) | |
Culture conditions | Static (7%), | Static (68%), | Static (50%), |
Rotation (93%) | Rotation (32%) | Rotation (50%) |