Fig.6

Skin wound healing in microstructure was improved by h-sEV-AT. a Representative images of the longitudinal section of the wounds on day 8 with H&E staining. The black inverted triangles pointed out the edge of the initial wound. The red inverted triangles pointed out the edge of wound areas/the edge of re-epithelization. The black dotted boxes represented areas that were magnified. In the right magnified panels, the black lines pointed out the area of the regenerated epithelium (ep.). Scale bar = 100 μm. The blue dotted boxes represented areas with immunohistochemical staining that were magnified in (b). b Representative CD31-stained images on day 8. The red arrows pointed out typical positive-stained blood vessels. Scale bar = 100 μm. c The number of CD31-stained blood vessels per field of view (scale bar = 100 μm) was analyzed (n = 4). d Representative images of the longitudinal section of the wounds at day 16 with Masson staining. The black dotted boxes represented areas that were magnified. In the right magnified panels, the red arrows pointed out regenerated hair follicles. Scale bar = 100 μm. e The number of hair follicles per field of view (scale bar = 100 μm) was analyzed (n = 4). The significance was tested with an unpaired two-tailed Student’s t test. ****p < 0.0001