Fig. 8

Macrophage phenotypes in skin wound healing. a Representative longitudinal section of the wounds at day 8 with immunofluorescence staining (green: F4/80, red: iNOS/CD206, blue: DAPI). The white dotted lines pointed out the edge of wound areas. The white dotted boxes represented areas that were magnified in the right-magnified panels. Scale bar = 50 µm. b The percentage of M1 macrophages and M2 macrophages in total macrophages per field of view (scale bar = 50 µm) was analyzed (n = 4). c The ratio of M2/M1 per field of view (scale bar = 50 µm) was analyzed by ImageJ software (n = 4). The significance was tested with an unpaired two-tailed Student’s t test. ***p < 0.001, ****p < 0.0001