Fig. 2

In vitro T-cell activity against dermal fibroblasts and isogenic iPSC-derivatives. A The allogenic T-cells (N = 9) co-cultured with isogenic dermal fibroblasts or iPS-fibro demonstrated increased expression of activation marker CD69 compared to autologous T-lymphocytes. The bars represent the mean ± SEM. **P < 0.01; ***P < 0.001; two-way ANOVA. B Number of allogenic CD69⁺ T-cells (N = 9) was greatly reduced against ΔiPS-fibro. The bars represent the mean ± SEM. The dots represent independent experiments with each donor's T-cells performed in triplicates. ***P < 0.001; RM one-way ANOVA. C Representative flow-cytometry plots illustrate CD69 expression for T-lymphocytes of an allogenic donor. T-cells cultured without target cell were used as negative control