Fig. 3

In vitro NK-cell response to dermal fibroblasts and isogenic iPSC-derivatives. A Autologous NK-cells demonstrated significantly higher degranulation against iPS-fibro and ΔiPS-fibro compared to dermal fibroblasts. The bars represent the mean ± SEM. ***P < 0.001; ordinary one-way ANOVA. B The degranulation index of allogenic NK-cells (N = 21) did not differ between iPS-fibro and ΔiPS-fibro. Each dot represents an independent experiment performed in triplicates. The dots represent the mean ± SEM. ***P < 0.001; RM one-way ANOVA. C Representative flow-cytometry histograms illustrate CD107a expression for NK-cells of an allogenic donor co-cultured with dermal fibroblasts and isogenic iPSC-derivatives. Degranulation against the K562 cells used as the positive control is indicated in gray. The estimates of the degranulation index are given in red. D The LDH release assay demonstrated NK-cell cytotoxicity against dermal fibroblasts and isogenic iPSC-derivatives. The bars represent the data on cytotoxicity of an allogenic donor's NK-cells mixed at the different effector/target (E/T) ratios. Each bar represents an independent experiment performed in triplicates. The bars represent the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; two-way ANOVA