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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: iPSC-derived cells lack immune tolerance to autologous NK-cells due to imbalance in ligands for activating and inhibitory NK-cell receptors

Fig. 5

Prolonged iPS-fibro cultivation promoted HLA-I upregulation but failed to inhibit NK-cell responses. A A gradual increase of HLA-ABC expression was observed during the process of passaging and maturation of iPS-fibro. HLA-ABC expression was normalized to isogeneic parental fibroblasts. The bars represent the mean ± SEM; ***P < 0.001; ordinary one-way ANOVA. B Prolonged passaging of iPS-fibro had no effect on the immune response mediated by autologous or allogeneic (N = 9) NK-cells. Each bar represents the mean ± SEM; **P < 0.01; ***P < 0.001; RM one-way ANOVA. C Expression of ligands for NKG2D activating receptor in parental fibroblasts and iPS-fibro at different passages. Expression of all analyzed genes in iPS-fibro was normalized to isogeneic parental fibroblasts. The bars represent the mean ± SEM; **P < 0.01; ***P < 0.001; RM one-way ANOVA. D Expression of ligands for DNAM-1 activating receptor in parental fibroblasts and iPS-fibro at different passages. Expression of all analyzed genes in iPS-fibro was normalized to isogeneic parental fibroblasts. The bars represent the mean ± SEM; **P < 0.01; ***P < 0.001; RM one-way ANOVA

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Stem Cell Research & Therapy

ISSN: 1757-6512