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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: iPSC-derived cells lack immune tolerance to autologous NK-cells due to imbalance in ligands for activating and inhibitory NK-cell receptors

Fig. 6

The pretreatment of iPS-fibro with IFNγ reduced the level of NK-cell degranulation and cytotoxicity. A Flow cytometry analysis revealed an increase in HLA-ABC expression in fibroblasts and iPS-fibro after IFNγ-stimulation (indicated in red). Isotype control is indicated in gray. B The autologous and allogeneic (N = 7) NK-cells demonstrated significantly reduced degranulation against iPS-fibro pretreated with IFNγ. Each bar represents the mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001; RM one-way ANOVA. C The LDH release assay demonstrated a reduction in NK-cell cytotoxicity against iPS-fibro pretreated with IFNγ. The bars represent data on the cytotoxicity of two allogenic donors’ NK-cells. Each bar represents an independent experiment performed in triplicates. The bars represent the mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001; ordinary one-way ANOVA

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Stem Cell Research & Therapy

ISSN: 1757-6512