Fig. 2

Angiogenesis properties of ELR-based hydrogel transplanted in a mouse model of limb ischemia (A–J). Bright-field imaging indicates the formation of de novo vessels within the ELR-based hydrogel on day 7. CD31⁺ vessels are indicated using immunofluorescence staining (A; Scale bar: 100 µm). Immunofluorescence staining revealed the formation of α-SMA⁺ arterioles and CD31⁺ vessels in ELR-treated mice compared to the PBS group (B; Scale bar: 20 µm). Mean capillary density (C; Scale bar: 20 µm); mean length density (D; Scale bar: 20 µm); stereological quantification (E; Scale bar: 20 µm); arteriole density (F; Scale bar: 20 µm); and mean the number of cross-sectioned arterioles (G; Scale bar: 20 µm). Proteomic analysis of ELR-treated group related to PBS mice (H-J). Heat map analysis of angiogenesis factors (I) and their relative upstream factors (J). n = 6; Two-way ANOVA analysis.*p < 0.05. Adapted from [273]. (2021; Biomaterials; https://doi.org/10.1016/j.biomaterials.2020.120641)