Fig. 4

Overexpression of KCNJ2 enhances Ca2+ signaling maturation in iPSC-CMs. a Representative Ca²⁺ transient tracings recorded by Fura-2 Ca2+ imaging from Vector OE (black) and KCNJ2 OE (red) iPSC-CMs under 0.5 Hz field stimulation. b Schematic diagram of Ca2+ transients demonstrating key parameters to analyze. Transient duration 90 denotes the full duration of a Ca²⁺ transient at 10% of maximal amplitude. Decay 90 denotes the difference in time from the peak of Ca²⁺ transient to the point when intracellular Ca²⁺ returns 90% from the maximal amplitude. c–h Scheme to bar graphs to compare key Ca²⁺ transient parameters between Vector OE (n = 34 cells) and KCNJ2 OE (n = 66 cells) iPSC-CMs, including transient amplitude, transient duration 90, decay 90, time to peak, maximal rising rate, and maximal decay rate