Fig. 6
RNA sequencing reveals distinct transcriptomic profile in KCNJ2 OE iPSC-CMs. a Bar graph showing the number of up- and downregulated differentially expressed genes (DEGs) between Vector OE and KCNJ2 OE iPSC-CMs. b Volcano plot showing the total of 205 DEGs. Red dots represent 95 upregulated DEGs. Green dots represent 110 downregulated DEGs. Gray dots represent non-DEGs. c Heat map demonstrating the differential gene expression pattern between Vector OE and KCNJ2 OE iPSC-CMs. d Top enriched Gene ontology (GO) analysis for cellular compartment (CC), molecular function (MF) and biological process (BP), respectively. e qPCR validation of a panel of genes associated with cardiac sarcomere proteins (TNNT2, ACTN2, MYBPC3, MYH7, MYH6 and MYL7), Ca2+ handing proteins (ATP2A2 and RYR2), and ion channels (SCN5A, CACNA1C, KCNH2 and KCNQ1). n = 3–4 independent differentiations