Fig. 7

Establishment of FTX+ /hDPSCs or FTX-/hDPSCs and the effect of FTX on cell proliferation. A–C The subcellular localization of FTX in hDPSCs was detected by fluorescence in situ hybridization (× 200). (A1, A2: The green fluorescence of the FTX probes; B1, B2: Nucleus stained with DAPI; C1, C2: Merged pictures). D FTX expression at different time points after FTX interference in hDPSCs. E Green fluorescence of FTX overexpression lentivirus in hDPSCs (× 50). F FTX expression after overexpression in hDPSCs. G, I The CCK-8 assay showed that the LPS stimulus significantly inhibited the proliferation of hDPSCs. Knockdown of FTX promoted cell proliferation, while overexpression of FTX further reduced the proliferation of hDPSCs in the LPS groups. H, J The EdU assay showed that FTX silencing conspicuously promoted the incorporation of EdU in the genome of LPS-induced hDPSCs, while upregulation of FTX notably decreased the EdU-positive cells. *: compared with the vector group, P < 0.05; **: compared with the vector group, P < 0.01; ∆: compared with the LPS stimulation group, P < 0.05; ∆∆: compared with the LPS stimulation group, P < 0.01