Fig. 9

Effect of FTX on the adipogenic differentiation of hDPSCs. A After 21 days of adipogenic induction, lipid droplet formation in hDPSCs was observed by oil red O staining (× 200). The LPS inflammatory stimulus reduced lipid droplet formation. DPSCs overexpressing FTX exhibited fewer and smaller lipid droplets than the vector groups. B The real-time PCR results showed that LPS reduced the expression of the adipogenic-related genes PPARγ-2 and LPL, and overexpression of FTX further decreased their expression in both the LPS-stimulated and nonstimulated groups. C The protein expression of PPARγ-2 and LPL in each group showed a similar trend to their gene expression. (C1: Representative bands of the protein expression; C2: Quantitative analysis of western blotting; Full-length blots are presented in Additional file 4). *: compared with the vector group, P < 0.05; **: compared with the vector group, P < 0.01; ***: compared with the vector group, P < 0.001; ∆: compared with the LPS stimulation group, P < 0.05; ∆∆: compared with the LPS stimulation group, P < 0.01; ∆∆∆: compared with the LPS stimulation group, P < 0.001