Fig. 5
Endocytosis of apoVs diminished H2O2-induced HEI-OC1 cells death and reduced cellular and mitochondrial ROS. a Representative fluorescence microscope images showing endocytosis of PKH26-labeled apoVs in HEI-OC1 cells (green) through co-culture. Scale bar = 50 μm. DAPI staining for nucleus. b Cell Counting Kit-8 (CCK8) assay examining the HEI-OC1 cell viability in each group. N = 6 per group. c flow cytometry assay of DCFH-DA showing the cellular ROS levels in HEI-OC1 cells of each group. d quantification of median fluorescence intensity of DCFH-DA confirming the significant difference of cellular ROS levels between each group. N = 4 per group. e representative fluorescence microscope images of MitoSOX staining (red) showing the mitochondrial ROS levels in HEI-OC1 cells of each group. Scale bar = 50 μm. DAPI staining for nucleus. f semi-quantification of MitoSOX fluorescence intensity in HEI-OC1 cells. N = 5 per group. Data are presented as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001