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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Apoptotic vesicles resist oxidative damage in noise-induced hearing loss through activation of FOXO3a-SOD2 pathway

Fig. 6

ApoVs upregulated SOD2 and promoted FOXO3a nuclear translocation. a, b Western-blotting analyzed the SOD2 expression in HEI-OC1 cells of each group (a), and statistical analysis (b). N = 8 per group. c RT-PCR analysis indicating the transcriptional levels of SOD2. N = 4 per group. d, e, f western-blotting of cytoplasmic and nuclear extracts from HEI-OC1 cells showing the expression of FOXO3a, GAPDH and Laminb1 (d), and statistical analysis (e, f). The expression of cytoplasmic (e) and nuclear (f) FOXO3a were relative to GAPDH and Laminb1 respectively. N = 4 per group. g representative fluorescence microscope images showing the location of FOXO3a protein in HEI-OC1 cells of each group. Scale bar = 50 μm. Phalloidin staining (red) illustrating the HEI-OC1 cells structure. DAPI staining (blue) for nucleus. Data are presented as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, NS, no significant difference

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Stem Cell Research & Therapy

ISSN: 1757-6512