Fig. 1

Proliferation and population doubling time of hUCPVCs (N = 3 donors, n = 3 technical replicates per donor) and BMMSCs (N = 3 donors, n = 3 technical replicates per donor) in xeno-free StemMACS™ and SCM. StemMACS™ culture medium supported the proliferation of both hUCPVCs and BMMSCs similarly to conventionally used SCM in an 8-day proliferation assay. Proliferation of hUCPVCs in each media formulation was similar to that of BMMSCs after 2 and 4 days in culture. After 6 and 8 days in culture, there were significantly more hUCPVCs than BMMSCs in both StemMACS™ (Day 6: p = 0.049; Day 8: p < 0.0001) and SCM (Day 6: p = 0.0033; Day 8: p < 0.0001) media conditions, suggesting a superior proliferative capacity of hUCPVCs. Inset: The average population doubling time of hUCPVCs was lower than that of their BMMSC counterparts in both StemMACS™ and SCM (p = 0.038), though this difference was only statistically significant in the latter media condition. Reported values are mean ± SEM; statistical analysis by two-way ANOVA with post-hoc Tukey’s multiple comparisons test. Dashed grey line indicates initial cell seeding density in a 6-well plate. Each shade corresponds to a different donor of hUCPVCs (Donor 0917003; Donor 0917004; Donor 0317004 from dark to light shade of blue) and BMMSCs (Donor 8013L; Donor 36341; Donor 36550 from light to dark shade of yellow/orange)