Fig. 2

A Frequency of CFU-F in hUCPVCs and BMMSCs (N = 3 donors each, n = 3 technical replicates per donor) cultured in StemMACS™ and SCM. Culture with StemMACS™ resulted in a similar frequency of CFU-F compared to culture with SCM for BMMSCs. hUCPVCs cultured in StemMACS™ had a significantly higher frequency of CFU-F than their counterparts cultured in SCM (p = 0.012). Reported values are mean ± SEM; statistical analysis by two-way ANOVA with post-hoc Tukey’s multiple comparisons test. Each shade corresponds to a different donor of hUCPVCs (Donor 0917003; Donor 0917004; Donor 0317004 from dark to light shade of blue) and BMMSCs (Donor 8013L; Donor 36341; Donor 36550 from light to dark shade of yellow/orange). B Crystal violet stained CFU-F colonies imaged under brightfield microscopy (scale bar = 1000 μm; inset scale bar = 500 μm). hUCPVCs cultured in StemMACS™ produced larger and more densely populated colonies than those cultured in SCM. BMMSCs consistently had smaller and less densely populated colonies than hUCPVCs in both media conditions