Fig. 6

F-actin (green) and nuclear (blue) staining of hUCPVCs cultured on electrospun PCNU for 14 and 21 days in StemMACS™. Confocal images and frequency distribution plot of F-actin alignment in hUCPVC-seeded PCNU scaffolds (N = 3) showed two principal angles of cell alignment: A hUCPVCs in direct contact with PCNU fibres aligned with the scaffold fibres (90°), whereas B hUCPVCs growing on a monolayer of cells away from the surface of the scaffold aligned away from the principal direction of the PCNU fibres. PCNU fibres oriented at 90° marked by a grey dashed line on frequency distribution plots. Confocal images of hUCPVC-seeded PCNU constructs are representative of N = 3 on day 14 (scale bar = 50 μm)