Fig. 7

Quantification of total and DNA-normalized hydroxyproline (OH-proline) (A–B), elastin (C–D), and sulphated glycosaminoglycans (s-GAG) (E–F) synthesized by hUCPVCs from a single donor cultured on electrospun PCNU (N = 3) for 14 and 21 days in StemMACS™ culture medium. Total A OH-proline, E s-GAG and G DNA content increased between 14 and 21 days in culture, although this change was not statistically significant. Elastin deposition after 14 days of culture was not measured (NM) and time-dependent changes in elastin content were not evaluated. DNA-normalized B OH-proline and D elastin content was similar to or approaching that on tissue culture polystyrene (Fig. 4B and D), while DNA-normalized s-GAG content was greater than in tissue culture polystyrene (Fig. 4F). H Movat’s pentachrome staining of hUCPVC-seeded PCNU constructs shows that the cells and deposited ECM grew in layers above the PCNU scaffold. Black arrows label boundary between hUCPVC and scaffold (scale bar = 50 μm). Reported values are mean ± SEM; statistical analysis by unpaired t-test