Fig. 2

Effect of Braco-19 on adipocyte differentiation of ASCs. ADIPO were obtained by differentiating ASCs in the presence and absence of Braco-19 (0.3, 0.6, 1.2, 2.5, 5 µM). Lipid accumulation was determined by Oil Red O staining. Representative microscopic images (× 10 magnification) are reported on the left. Bar graph (on the right) shows Oil Red O quantification as percentage of Oil Red O levels in the presence of Braco-19 compared to untreated cells, considered as maximum differentiation (100%). Data (mean of at least three independent experiments) were analyzed using one-way ANOVA for multiple comparison with Dunnett method (*p < 0.05; **p < 0.01) (A). FSC and SSC were analyzed within the flow cytometry acquisitions of the cell cycle for ASCs, ADIPO and ADIPO treated with Braco-19 (2.5 or 5 µM). Data are reported as boxplots (mean of three independent experiments) showing the mean fluorescence intensity (MFI) as fold-change of ADIPO compared to ASCs (left graph), and ADIPO treated with Braco-19 compared to untreated ADIPO (right graph) (B). Statistical analysis was carried out by one unpaired T test per row correct for multiple comparisons using the Holm–Sidak method (left panel, *p < 0.0001; right panel,*p < 0.05;**p < 0.00001)