Fig. 4

Effect of Braco-19 on fibro-senescence features in adipocyte differentiated from ASCs. ASCs were differentiated toward the adipogenic lineage (ADIPO) in the presence and absence of Braco-19 (2.5 or 5 µM). At the end of the process, blue Sa-b-Gal-positive senescent cells were detected by microscopy (× 10 magnification). Images are representative of at least three independent experiments (A). mRNA levels of senescence and fibrosis markers LMNB1 (B), ACTA2 and FSP1 (C) were determined by qPCR. mRNA levels were normalized on PPIA gene as internal standard. Results are represented as mean ± SD of three independent experiments and show LMNB1, ACTA2 and FSP1 expression levels in cells treated with Braco-19 relative to those in untreated cells