Fig. 1

Algorithm identification of square-shaped nanowells. A Illustration of a CellCelector 24-well nanowell plate. Each nanowell forms a square (200 × 200 µm) delimited by 100 µm height walls. Well diameter = 1.6 cm. B Automated segmentation of nanowells in the automatically stitch whole well image. In each representative well, the software identifies by segmentation 2263 square-shaped nanowells (1 mm peripheral region of the well is not analyzed). Each identified nanowell is surrounded by red lines and assigned a unique ID for traceability through the experiment. Insert corresponds to magnified area. Scale bar = 2 mm and for magnification = 200 µm. C. Overview of the CellCelector™ system: (a) inverted fluorescence microscope and motorized stage; (b) platform to receive destination plates and (c) cell picking robotized arm with high-precision glass capillary (magnification in D). E Upper view of the robotic system: the platform is composed of dedicated areas: (a) destination plates, (b) sterilization tank and (c) waste container