Fig. 2

Automated isolation of single cell-derived clones in nanowells. A Schematic representation of the automated isolation of single cell-derived hPSC clones. A mixed population of label-free, mCherry- and GFP-labeled hiPSCs are mixed (1:1:1) prior single cell seeding into nanowell plates and automated clone picking. B Representative images of label-free hiPSCs, GFP-hiPSCs and mCherry-hiPSCs seeded as single cells at day 0 (D0; a), at 3 days (D3) of culture within nanowells (b), after enzymatic treatment to harvest clones (c) and after picking by the automated arm of the CellCelector™ (d). The selected square-shaped micro-pattern for picking is at the center of the image. The picked single cell-derived clone is highlighted with dotted lines in panels (c) and (d). Following picking, colonies are deposited into a 96-well plate where their growth is followed by imaging at D5, D10 and D18 (e, f, g). Scale bar in 24-well plate images = 100 µm; scale bar in 96-well plate images = 500 µm