Fig. 5
From: Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones
Single cell-derived clones CRISPR/Cas9 edited remain pluripotent and without genomic aberrations. A Immunofluorescence images of hPSC colonies (WT before selection and p.Glu192fs purified clone) stained for pluripotency markers OCT3/4 (red) and LIN28a (green). Scale bar = 50 µm. B Karyotype of a p.Glu192fs purified clone. C Flow cytometry analysis of pluripotency markers TRA1-81 and SSEA-4 on WT before selection (upper panel) and p.Glu192fs hPSCs (lower panel). D Immunofluorescence images of hPSCs stained for Pericentrin (red) and ALMS1 (green). Scale bar = 5 µm