Fig. 1
gmfg is a potential regulator of HSPC development. a Venn diagram showing the upregulated DEGs in the PSC-, FL- and BM-derived HSPCs as compared to undifferentiated PSCs (172 common upregulated DEGs). b Top 20 statistically enriched pathways of the common upregulated DEGs in the PSC-, FL- and BM-derived HSPCs. c gmfg expression in human tissues. The abscissa represents different human tissues, the ordinate represents gene expression level, and the histogram represents the expression level of gmfg in specific human tissue. Black arrow denotes gmfg expression in CD34 + cells. d Dynamic analysis of gmfg, CD34, CD43 and CD45 expression with real-time PCR during hESC hematopoietic differentiation. Relative expression is normalized by undifferentiated hESCs (except for CD45 by differentiated hESCs at D9). Data are shown as mean ± SD (n = 3). ns, not significant; *p < 0.05, ***p < 0.001, ****p < 0.0001. e Quantitative RT-PCR analysis of gmfg expression in undifferentiated hESCs, mesoderm (CD309 +), HEPs (CD31 + CD34 +), and HPCs (CD43 + and CD45 +) derived from hESCs. Relative expression was normalized by undifferentiated hESCs. Data are shown as mean ± SD (n = 3). ***p < 0.001, ****p < 0.0001. f Quantitative RT-PCR analysis of gmfg expression in hESCs treated with ctl-sh, gmfg-sh2, and gmfg-sh3. Data are shown as mean ± SD (n = 3). ***p < 0.001, ****p < 0.0001. g Flow cytometry analysis of CD31 + CD34 + HEPs and CD43 + HPCs generated from ctl-sh, gmfg-sh2, and gmfg-sh3 hESCs at day6 and day9 of differentiation, respectively. Data are shown as mean ± SD (n = 3). **p < 0.01, ****p < 0.0001. h Expression pattern of gmfg during zebrafish embryogenesis: The stage examined by whole-mount in situ hybridization (WISH) is shown in each panel: 1‐cell stage, 2‐cell stage, 4 h postfertilization (hpf), 13 hpf (Scale bars: 200 μm), 24 hpf, 36 hpf, 48 hpf and 72 hpf (Scale bars: 100 μm). i (left) Graphic representation of the sorting strategy of kdrl + cmyb- ECs and kdrl + cmyb + HE from kdrl:mCherry;cmyb:GFP transgenic zebrafish by FACS at 48 hpf. (right) Quantitative RT-PCR analysis of gmfg and gmfb expression in FACS-sorted kdrl + cmyb- ECs and kdrl + cmyb + HE. Bars represent mean ± SD (n = 3). ns, not significant; **p < 0.01