Fig. 2

Loss of gmfg, but not gmfb, induces HSPC defects. a WISH results of runx1 and cmyb in the AGM in control and gmfg morphants at 36 hpf. The red arrowheads indicate the expression of runx1 and cmyb. b Western blotting showing the protein level of Runx1 and Cmyb in control and gmfg morphants at 36 hpf. Representative blot is shown in the figure (Full-length blots are presented in Additional file 2: Fig. S1 and S2). Data represent mean ± SEM intensity of indicated blots (n ≥ 3). *p < 0.05, ***p < 0.001, ****p < 0.0001. c WISH analysis showing the expression of runx1 (red arrowheads) in the DA in control and gmfg morphants at 24 and 28 hpf. d WISH analysis showing runx1/cmyb expression (red arrowheads) in the DA in control, gmfg-atgMO, and coinjection of gmfg-atgMO and gmfg mRNA embryos at 28 hpf. e WISH analysis showing the expression of cmyb (red arrowheads) in the DA in embryos injected with control MO and gmfb-MO at two different doses (gmfb-MO1: 9.6 ng and gmfb-MO2: 12.8 ng per embryo) at 30 hpf. f Representative images showing runx1 expression (red arrowheads) in the DA in control, gmfg-atgMO and gmfb-MO2 (12.8 ng per embryo) at 24 hpf. g Maximum projections of 48 hpf kdrl:mCherry; cmyb:GFP double-transgenic embryos injected with control MO, gmfg-atgMO, and gmfg-spMO. Arrowheads denote kdrl + cmyb + HE along the DA. All views: anterior to left. h Enumeration of kdrl + cmyb + HE shown in (g). Bars represent mean ± SD of control MO (n = 10), gmfg-atgMO (n = 21), and gmfg-spMO (n = 8). ***p < 0.001. Numbers at the lower right corner of the picture represent embryos with displayed phenotype/whole embryos. All scale bars, 100 µm