Fig. 2

Functional measurements of mitochondrial activity in rescued tenocytes. A ΔΨm was determined by confocal microscopy using JC-1 dye (n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in mPTP opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI (n = 3). D Quantitative analysis of ATP using the ATP determination kit (n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content (n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 (n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001