Fig. 2

MenSCs decreased the number of activated microglia cells and attenuated 6-OHDA induced inflammation in vitro. Iba-1 was used to mark BV2 (A1–C3) and HAPI cells (E1–G3) using immunofluorescence. A1–G1 The morphology of BV2 and HAPI cells was observed and captured under bright field. A2–G2 Cell nucleus were stained with DAPI. A3–G3 The expression of Iba-1. D, H the percentage of activated BV2 cells (D) and HAPI cells (H). I, J Pro-inflammatory and anti-inflammatory factors in BV2 and HAPI cells were detected by qRT-PCR. One-way ANOVA followed by Tukey’s post hoc test was used for statistical analysis. ^nsp > 0.05, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001