Fig. 4

The expression of TH in the SN, VTA and striatum regions. A–C The expression of TH in the VTA and SN regions was detected by immunohistochemistry. D–F TH positive fibers in the striatum region was detected by immunohistochemistry. G–I statistical analysis of TH positive neurons and fibres. G and H showed the fold change of TH positive neurons in right SN/VTA regions compared to that in contralateral side. Image J software was used to count the number of TH positive neurons in the SN and VTA regions. I showed the relative density of TH positive fibers in right striatum regions compared to that in contralateral side. J The expression level of TH in brain was detected by WB. Full-length blots were presented in Additional file 3: Fig. S2 D, E. K Statistical analysis of WB results. Abbreviations: SN, substantia nigra; TH, tyrosine hydroxylase; VTA, ventral tegmental area. One-way ANOVA followed by Tukey’s post hoc test was used for statistical analysis. ^**p < 0.01, ^***p < 0.001, ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001