Fig. 6

The expression of M1 and M2 microglial markers in rat brain detected by qRT-PCR and WB. A qRT-PCR was used to detect markers of M1 phenotype microglia including CD11b, IL-1β, iNOS, MHCII and TNF-α. B qRT-PCR was used to detect markers of M2 phenotype microglia including Arg1, CD206, IL-10, and YM1/2. C WB results showed the represent cropped images of Arg1, iNOS, and internal control β-actin. Full-length blots were presented in Additional file 3: Fig. S2 A-C. D Statistical analysis of the WB results of Arg1 and iNOS. One-way ANOVA followed by Tukey’s post hoc test was used for statistical analysis. ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001