Fig. 7

Overexpression of miR-199a-5p in Thy1-MC EVs. Administration of Thy1-MC EVs and cells transfected with miR-199a-5p to Ret/PH-treated rat livers. EVs obtained from the conditioned medium of cultured Thy1-MCs transfected with miR-125b-5p and miR-199a-5p for 48 h were administered, and the livers were examined 14 days later. A Quantification of miR125b-5p and miR-199a-5p expression in Thy1-MC EVs with and without transfection via qRT–PCR. Bars indicate SEs. Asterisks indicate significant differences at p < 0.05. B Photos of SHPC clusters in the livers of control, EVs secreted by Thy1-MCs transfected with negative control (NT), or miR-199a-5p (miR-199a). The samples were stained using HE. SHPC clusters are enclosed by dotted lines: scale bar, 200 μm. C Number of SHPC clusters in each liver area and D number of cells per cluster are shown. Bars indicate SEs and asterisks indicate significant differences at p < 0.05