Fig. 3

SOCS3 blocks the improvement in islet function following pre-culture with SDF-1 in NOD mice. In vivo graft function of SDF-1 pre-cultured SOCS3-KO islets. A Blood glucose concentrations of mice transplanted with 200 islets or SOCS3-KO islets pre-cultured alone or with 10 nM/L SDF-1 for 2 days. SOCS3 KO islets + SDF-1 group showed significantly lower glucose levels compared to those in the other groups (repeated measurement of ANOVA with Bonferroni post hoc test, n = 6). B Kaplan–Meier Log-rank test of STZ-treated NOD recipients maintaining islet graft function based on blood glucose readings (< 16.7 mM/L) after transplantation with 200 SOCS3-KO islets under the kidney capsule with or without SDF-1 (n = 9 per group). C Double immunofluorescence staining for DAPI (blue) and insulin (green) at 7 days post-transplantation in SOCS3 KO islets and SOCS3 KO islets + SDF-1 groups. Magnification: × 200. D Intraperitoneal glucose tolerance tests (IPGTTs) in all cured mice at 1 month after transplantation as above (repeated-measurement ANOVA with Bonferroni post hoc test, n = 3–5). E Two-way ANOVA with multiple comparisons for the area under the curve of the IPGTT. ns = not significant; *p < 0.05