Fig. 5

SDF-1 preconditioning suppressed inflammatory responses in the grafted SOCS3-KO islets. SOCS3-KO islet allografts with or without SDF-1 were excised from STZ-treated NOD mice and gene expression and flow cytometry were performed (day 7). Graft IFN-γ, IL-2, and IL-6 mRNA levels were quantified by qRT-PCR (A), and protein concentrations in recipient mouse sera were determined by ELISA (B). Flow cytometry analysis of the frequency of arginase-1⁺ CD11c⁺ DCs (C), arginase-1⁺ F4/80⁺ macrophages (D), MHC-II⁺ CD11c⁺ DCs (E), and MHC-II⁺ F4/80⁺ macrophages (F) (n = 5). Data represent three independent experiments. *p < 0.05