Fig. 4

Spheroid cells showed improved tumor forming ability in the in vivo xenograft study. A, B Xenograft tumor growth of nude mice injected with spheroid cells and parent cells of Ishikawa (n = 3 per group, A) or HEC1A cells (n = 6 per group, B). The volume C, D and weight E, F of xenografted tumors transfected with Ishikawa parent cells and spheroid cells were measured and analyzed, respectively. Tumor volume was calculated every three days after injection, and the tumor was excised and weighed after 21 days and 23 days. Bar graphs display mean ± SD (* P < 0.05 vs. parent cells, ** P < 0.01 vs. parent cells, *** P < 0.001 vs. parent cells)