Fig. 1

Representative images of the gating strategy for the CD34+ population and cell viability using 7-AAD staining. Initially, leukocytes were gated using CD45+ to exclude platelets, red blood cell debris, and aggregates (first panel). Subsequently, viable CD45+ cells were identified as 7-AAD negative (second panel), followed by the identification of the CD34+ population (third panel). The fourth panel displays CD45 dim-positive cells, and the final panel indicates the identification of the true hematopoietic stem cell population (CD34+, CD45+)