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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Intravenous injection of human umbilical cord-derived mesenchymal stem cells ameliorates not only blood glucose but also nephrotic complication of diabetic rats through autophagy-mediated anti-senescent mechanism

Fig. 9

Effects of hucMSCs on cell senescence, mRNA levels, and protein expressions of podocytes under co-culture conditions. A and B Representative images of SA-β-gal staining on rat podocytes and the percentage of SA-β-gal-positive cells in each group (scale bar = 20 μm). C and D qPCR was performed to detect the expressions of senescence-related genes, including senescence markers p21and p53. (E) WB was performed to measure the expressions of autophagic pathway and senescence-related proteins, including p-AMPK, p-mTOR, p16, and p53. Full-length blots of rat podocytes were presented (Additional file 2: Figs. S8–S11, Additional file 3: Figs. S30–S33), respectively. Data were shown as mean ± SD, n = 3, unpaired, student's t test. *P < 0.05 and **P < 0.01 versus the normal group, #P < 0.05 and ##P < 0.01 versus the model group

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Stem Cell Research & Therapy

ISSN: 1757-6512