Table 1 Primer sequences used for qPCR analysis
Gene | Forward primer | Reverse primer |
|---|---|---|
GAPDH | 5'-GACATGCCGCCTGGAGAAAC-3' | 5'-AGCCCAGGATGCCCTTTAGT-3' |
p16 | 5'-TCCTTGGCTTCACTTCTGGCAAC-3' | 5'-TCCTTGGCTTCACTTCTGGCAAC-3' |
p21 | 5'-GAAAACGGAGGCAGACCAG-3' | 5'-TTCAGGGCTTTCTCTTGCAG-3' |
p53 | 5'-GTCTACGTCCCGCCATAAAA-3' | 5'-AGGCAGTGAAGGGACTAGCA-3' |
pRb | 5'-CTTGCGGATTCCTGGAGGTAACATC-3' | 5'-TCCCAAATGATTCACCGATTGAGACC-3' |
p62 | 5'-GGTGTCTGTGAGAGGACGAGGAG-3' | 5'-TCTGGTGATGGAGCCTCTTACTGG-3' |
Beclin-1 | 5'-TCAAGATCCTGGACCGAGTGACC-3' | 5'-CTCCTCTCCTGAGTTAGCCTCTTCC-3' |
LC3B | 5'-GAGCGAGTTGGTCAAGATCATCCG-3' | 5'-GATGTCAGCGATGGGTGTGGATAC-3' |
ULK1 | 5'-TACACAGCAAGGGCATCATTCACC-3' | 5'-CGGGCAAATCCAAAGTCAGCAATC-3' |