Fig. 7

The results and analyses of qPCR and western blot revealed that the conditional medium of MSCs with IronQ reduced mRNA and protein expression of Mincle and inflammatory cytokines. A–D The presentation of graphs for A Mincle, B IL-6, C TNF-α, and D IL-1β mRNA relative expression in different cells groups after 6 h (n = 3 for each group; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001). E–J showed that the results and analyses of western blot of IL-6, TNF-α, Mincle, and p-syk in the LPS-induced BV2 cell line. E Representative immunoblot showing the IL-6, TNF-α, Mincle, syk, p-syk, p-NFκB-p65, NFκB-p65, and GAPDH protein expression; (F-J) The quantitative densitometric ratio of F IL-6, G TNF-α, H Mincle, relative to GAPDH, I p-syk relative to syk and J p-NFκB-p65 relative to NFκB-p65 (n = 3 for each group; *P < 0.05, **P < 0.01, and ****P < 0.0001)