Fig. 2
MT relieved the inhibitory effect of MP on BMSC. A The structural formula of melatonin and CCK-8 analysis were performed with various concentrations of MT in BMSC for 24 h, 48 h, and 72 h. B ROS detection with 100 µM MP and various concentrations of MT treatment. (Scale bar: 100 μm) C PI staining. (Scale bar: 100 μm) (D) Mean fluorescence intensity of ROS. E Number of PI (+) cells. F qPCR analysis of the Gpx4, Sod2, Hmox1 and Nox4 genes in BMSC. G ALP staining and quantitative analysis. (Scale bar: 100 μm) H ARS staining and quantitative analysis. (Scale bar: 100 μm) I qPCR analysis of Runx2, Sp7, Alpl and Bglap expression in BMSC. (β-actin was selected as an internal reference.) J Flow cytometry analysis of ROS staining. K Flow cytometry analysis of PI staining. L Mean fluorescence intensity of ROS and PI staining. (All bar graphs are the mean ± SD. *p < 0.05, **p and & p < 0.01 compared with the MP 100 µM group. #p < 0.05 and ##p < 0.01 compared with the control group.)