Fig. 7

MT alleviated MP-induced ferroptosis through the melatonin MT2 receptor. A, B ROS detection. (Scale bar: 100 μm) C, D PI staining and analysis. (Scale bar: 100 μm) E–H Western blot analysis of SLC7a11, GPX4 and GDF15 in BMSC after treatment with or without 10 μM luzindole, 100 μM MP and 100 μM MT. (β-actin was selected as an internal reference.) I Flow cytometry analysis of mitochondrial potential by JC-1. (Excitation: 585 nm, emission: 590 nm) J Quantitative analysis of JC-1. K Detection of MDA. L Detection of total GSH. M Detection of the GSH/GSSG ratio. N Detection of the NADPH/NADP + ratio. (*p < 0.05 and **p < 0.01 compared with the 100 μM MP treatment group. ^#p < 0.05 and ^##p < 0.01 compared with the 100 μM MT treatment group. Full-length blots are presented in Additional file 2: Figures S9–S11.)