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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Exogenous melatonin ameliorates steroid-induced osteonecrosis of the femoral head by modulating ferroptosis through GDF15-mediated signaling

Fig. 8

MT alleviated MP-induced ferroptosis through the GDF15 signaling. A Flow cytometric analysis of PI staining. B Quantitative analysis of PI (+) cells. C Flow cytometry analysis of mitochondrial potential by JC-1 and quantitative analysis. (Excitation: 585 nm, emission: 590 nm) D MDA detection. E, F qPCR analysis of Slc7a11 and Gpx4 expression in BMSC. (β-actin was selected as an internal reference.) G ALP staining. (Scale bar: 100 μm) H Quantitative analysis of ALP activity. I ARS staining. (Scale bar: 100 μm) J Quantitative analysis of ARS. (*p < 0.05 and **p < 0.01 compared with the 100 μM MP treatment only group. #p < 0.05 and ##p < 0.01 are comparisons between 100 μM MP and 100 μM MT treatment groups after Gdf15 knockdown.)

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Stem Cell Research & Therapy

ISSN: 1757-6512