Table 1 Sequences of primers used for real-time qPCR analysis
Gene | Forward primer (5’‒3’) | Reverse primer (5’‒3’) |
|---|---|---|
Slc7a11 | TCCTTTCAAGGTGCCTCTG | TCCGAGTAAAGGGAGAGGA |
Acsl4 | AGTACAACTTTCCGCTTGTG | AAGCCTCAGACTCATTTAATCC |
Gpx4 | AGCAAGATCTGTGTAAATGGG | TTTGATGGCATTTCCCAGC |
Actb | TCAGGTCATCACTATCGGCAAT | AAAGAAAGGGTGTAAAACGCA |
Sod2 | GAACCCAAAGGAGAGTTGC | CACAGCTGTCAGTTTCTCC |
Hmox1 | AGGAACACAAAGACCAGAG | CAGAGGTAGTATCTTGAACCAG |
Nox4 | TCTTCTGGTATACTCACAACCT | GATACTTCAACAAGCCACCC |
Runx2 | GAACCAAGAAGGCACAGAC | AATGCGCCCTAAATCACTG |
Sp7 | CTGCTTGAGGAAGAAGCTC | TATGGCTTCTTTGTGCCTC |
Alpl | CTAGACACAAGCACTCCCA | CTAAGAGGTAGTCCACCCTG |
Bglap | GAATAGACTCCGGCGCTACC | TCCTGGAAGCCAATGTGGTC |