Fig. 3
From: Deacetylation of FOXP1 by HDAC7 potentiates self-renewal of mesenchymal stem cells
Knockout of FOXP1/HDAC7 exacerbates bone mass loss. a Representative dorsal view of 3-month-old Foxp1fl/fl, Foxp1Prx1∆/∆, HDAC7+/−and Foxp1Prx1∆/∆;HDAC7+/− double KO mice. b Western blot detection of FOXP1 and HDAC7 within BM MSCs of mice at 3 months of age. c qPCR validation of mRNA level of FOXP1 and HDAC7 in MSCs from mice at 3 months of age. Data shown are representative of 3 independent assays. d 3D view of cortical and trabecular bone structure by µCT analysis of femur bones of mice at 3 months of age. e–f Quantification of cortical and trabecular bone properties by µCT analysis (n = 6). Abbreviations: BV/TV, bone volume/tissue volume; BMD, bone mineral density; Tb. N., trabecular bone number; Tb. Sp., trabecular bone spacing; Tb. Th., trabecular bone thickness; BS, bone surface; Cb. Th., cortical bone thickness. Two-tailed Student's t tests for comparisons between two groups. Each group consists of 9 mice of each phenotype. g Crystal purple staining of CFU-F colonies of MSCs from 3-month-old mice (n = 3) of the indicated genotypes.*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ns, non-significant