Fig. 4
From: Deacetylation of FOXP1 by HDAC7 potentiates self-renewal of mesenchymal stem cells
FOXP1/HDAC7 deficiency impairs the self-renewal potency of MSCs a Population doubling curve of BM MSCs of Foxp1fl/fl, Foxp1Prx1∆/∆, HDAC7+/−, and Foxp1Prx1∆/∆;HDAC7+/− 3-month-old transgenic mice. Each group consists of 3 mice of each phenotype. b SA-β-gal staining (left panel) and qPCR analysis (right panel) for cellular senescence markers at passage 5 of MSCs in transgenic mice of each genotypes (n = 3) indicated in panel A. Bar, 200 μm. c Immunofluorescence of LAP2 (red) and DAPI (blue) for passage 5 MSCs from transgenic mice (n = 3) of genotype indicated in (a). Right panel shows the quantification of LAP2-positive cells. Bar, 100 μm. d Immunofluorescence of γH2AX (Green) and DAPI (blue) for passage 5 MSCs from knockout mice (n = 3) of each genotype indicated in (a). Right panel shows the quantification of γH2AX-positive cells. Bar, 100 μm. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ns, non-significant. e Detection of LAP2 and P16 protein expression in passage-5 MSCs from bone marrows of Foxp1fl/fl, Foxp1Prx1Δ/Δ, HDAC7+/−, and Foxp1Prx1Δ/Δ;HDAC7+/− mice (n = 3)