Fig. 5
From: Deacetylation of FOXP1 by HDAC7 potentiates self-renewal of mesenchymal stem cells
FOXP1/HDAC7 retroviral-mediated overexpression enhances the expansive potency of hMSCs. a Population doubling curves of hMSCs infected with retroviral pMSCV-FOXP1 and/or pMSCV-HDAC7. Human (h)MSCs were infected with pMSCV empty vector as a control. Data shown are representative of 3 independent replications. b qPCR analysis of p16/p21 expression in hMSCs overexpressing FOXP1/HADC7-. n = 3. Data shown are representative of 3 independent replications. c SA-β-gal staining at passage 5 of FOXP1/HDAC7 retroviral overexpressing hMSCs. Right panel, quantification of percentage of SA-β-gal-positive cells. Bar, 200 μm. d Immunofluorescence of LAP2 (red) and DAPI (blue) of passage 5 MSCs from mice (n = 3) as indicated. Right panel, quantification of LAP2-positive cells. Bar, 100 μm. e Immunofluorescence of γH2AX (Green) and DAPI (blue) for passage 5 MSCs from mice (n = 3) as indicated. Right panel, quantification of γH2AX-positive cells. Bar, 100 μm. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ns, non-significant