Fig. 3

CD69 elevation is dose-dependent at higher concentrations of LPS. A Representative FACS plots showing the percentage of CD69⁺ cells in the LSK (upper panels) and LSKCD48⁻150⁺ HSCs (lower panels) populations from the BM of PBS-treated (control) mice and mice stimulated with various doses of LPS (0.0001–20 µg) at 2 h post-injection. B, C Quantification of the LSK CD69⁺ and LSKCD48⁻150⁺ HSCs CD69⁺ cell populations from the BM of PBS-treated (control) mice and mice treated with different doses of LPS. D, E Linear regression model fitting the dose-dependent response with the expression of CD69 in LSK and LSKCD48⁻150⁺ HSCs populations. Solid lines represent the linear fit of data. Dotted lines represent 95% confidence intervals. Data are presented as average ± SD, a summary of three independent experiments, n ≥ 3 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001