Fig. 4

CD69 and CD317 expression on activated stem and progenitor populations positively correlate with exit from quiescence. A Representative FACS plots showing cell cycle analysis using DAPI and intracellular expression of Ki67 in LSK CD317⁻ (left panel) and LSK CD317⁺ (right panel) cells from pIpC-stimulated mice at 4 and 24 h after injection. B, C Quantification of cell cycle phases (G0, G1, S, and M) in CD317⁻ and CD317⁺ fractions of the LSK and LSKCD48⁻150⁺ HSCs populations from pIpC-stimulated mice at 4 h and 24 h post-injection. D Representative FACS plots showing cell cycle analysis using DAPI and intracellular expression of Ki67 in LSK CD69⁻ (left panel) and LSK CD69⁺ (right panel) cells from LPS-stimulated mice at 2 and 24 h after injection. E, F Quantification of cell cycle phases (G0, G1, S, and M) in CD69⁻ and CD69⁺ fractions of the LSK and LSKCD48⁻ CD150⁺ HSCs populations from LPS-stimulated mice at 2 h and 24 h post-injection. Data are presented as average ± SD, a summary of three independent experiments, n ≥ 3 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001