Fig. 3

No endogenous NSCs detected within the brainstem stroke. At 3, 7, 14, and 28 days after the onset of stroke, brain sections were stained with anti-Ki67 (green), anti-nestin (red), and DAPI (blue) to detect NSCs. A The number of endogenous NSCs in the ipsilateral SVZ was significantly increased when compared to the contralateral side at any time point. B NSCs were not detected in brainstem lesions at any time point. Infarct is marked by dotted line. C Histogram shows the number of NSCs in the SVZ. *P < 0.05, versus contralateral side, # versus Day 3, & versus Day 7, and % versus Day 14. N = 6, scale bar = 50 μm