Fig. 4

Low survival rate of grafted NSCs in the brainstem stroke model. A GFP-NSCs (green) were transplanted 1 day (T-Day 1 group), 3 days (T-Day 3 group), 7 days (T-Day 7 group), and 14 days (T-Day 14 group) after stroke onset. Brains were harvested on Day 1, 3, 7, 14, and 28 after transplantation. TUNEL staining (red) was used to detect apoptotic cells. B The schematic diagram of time points and groups. C The line chart showed the survival rate of the transplanted GFP-NSCs. Survival rate = \(\frac{{{\text{No}}.\;{\text{of}}\;{\text{GFP}} + {\text{cells}}\;{\text{on}}\;{\text{Day}}1 - {\text{No}}.\;{\text{of}}\;{\text{TUNEL}} + {\text{GFP}} + {\text{cells}}}}{{{\text{No}}.\;{\text{of}}\;{\text{GFP}} + {\text{cells}}\;{\text{on}}\;{\text{Day}}1}} \times 100\). *P < 0.05, versus T-Day 1, #P < 0.05, versus T-Day 3, &P < 0.05, versus T-Day 14. N = 6, scale bar = 20 μm